In a related study aimed to elucidate the effects of two soy anti nutrients, saponins and phytosterols, on growth, intestinal health and function of European sea bass juveniles was studied. Seven fishmeal-based diets were formulated with varying levels of soy saponins and phytosterols. After 59-days, blood was collected for plasmatic cholesterol quantification and intestinal samples were collected for histology, digestive enzymes activity and gene expression analysis. Feeding low levels of phytosterols resulted in increased distal intestine weight and decreased plasma cholesterol, while feeding high levels of phytosterols caused inflammatory changes in the distal intestine. Feeding high levels of saponins depressed some enzyme activity in distal intestine and the histological inflammatory changes. This study indicated that feeding saponins and phytosterols at levels tested did not compromise growth, however, caused some gastrointestinal disturbance that may affect fish in the long term.