Enspira (ESP) and Enspira+ (ESP+) are precision engineered blends of exogenous enzymes designed to degrade non-starch polysaccharides in common diet formulations. An experiment was conducted to determine the response of apparent and standardized ileal digestibility (SID) of amino acids (AA) in soybean meal to ESP or ESP+. Cobb-500 male broiler chickens were tagged, placed in battery cages, and fed a corn-soybean meal (SBM)-based starter diet. At 23 d of age, birds were individually weighed and 272 birds were allotted by body weight to 4 dietary treatments in a randomized complete block design. An N-free diet (10 birds/cage) and 3 corn starch-dextrose based semi-purified diets (8 birds/cage), formulated to contain 407.7 g/kg of SBM as the sole source of dietary N, were prepared, and 5 g/kg of TiO2 was added as an indigestible marker. Enspira or ESP+ was added at 125 mg/kg to two SBM-containing diets, respectively, at the expense of dextrose. At 27 d of age, birds were euthanized, weighed, and digesta was collected from the terminal two-thirds of the ileum. Feed and digesta samples were dried and analyzed for dry matter, N, Ti, and AA content. The SID was determined by correcting the apparent ileal AA digestibility for the basal ileal endogenous AA losses determined using the N-free diet. Adding ESP or ESP+ increased (P < 0.05) the SID from 89 to 95% and 92 to 93% for Lys and Thr, respectively, as well as Arg, His, Ile, Leu, Ala, Ser, Asp, and Gly. None of the 2 enzyme blends had effect on the SID of Met, Val, Trp, Cys, or Tyr. Enspira+ reduced the SID of Phe (P < 0.05).
These data indicate that including 125 mg/kg of ESP or ESP+ improves or has no detrimental effect on the SID of most of the indispensable and dispensable AA in SBM.