Exogenous enzyme treatment is commonly added to poultry diets to improve nutrient utilization, reduce excretion of nutrients into the environment, improve animal/bird performance and to reduce the cost of production. A series of four experiments were conducted in broilers to determine the efficacy and tolerance to an enzyme product (Vegpro™) containing protease and xylanase activities in wheat-soybean meal diets fed to broilers from 1 to 39 or 42 days of age. The experiments tested protease activity levels of 0, 2,500, 5,000, 10,000, 20,000, 30,000, 40,000 or 100,000 HUT/kg of feed. Results indicated that Vegpro™ improved average daily gain and feed conversion ratio, especially in birds up to 21 d of age. Overall, inclusion of Vegpro™ to broiler diets at 10,000 HUT protease per kg of feed was shown to provide the maximum weight gain and feed conversion benefits. Increasing the protease activity to100,000 HUT/kg, (ten times the recommended inclusion rate) was well tolerated and did not adversely affect the measured health and performance indicators from 1 to 42 d of age.